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10.1073/pnas.1713845115 8 BersukerK.ClarkW

et al., 2021) demonstrated that the administration of metformin changed the relative abundances of metabolites such as carbohydrates, amino acids, and fatty acids as well as specific gut bacteria ( Escherichia, Romboutsia, Intestinibacter, and Clostridium )

After 4-day culture, organoids were dissociated in organoid dissociation solution (bioGenous, E238001) for 2 min at 37 C according to the manufacturers protocol, centrifuged at 68 g for 2 min, resuspended in organoid culture medium, and cultured as described above for organoids passaging

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