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Immunoblotting test was used to validate the results of proteomic approach

Ca 2+ signaling plays a pivotal role in regulating neuronal activity, and transient increases in cytoplasmic Ca 2+ activate a variety of important calcium-dependent cellular events, including both the CaMK and PKA pathways ( in vivo 12 h after BHBA administration (data not show), it might due to the highly dynamic property of CaMKII phosphorylation and dephosphorylation

The other side is renewal: fasting, autophagy, cellular cleanup, clearing out damaged cells

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