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Aerosol and e-liquid concentrations were both determined at the same point and analysed as part of the same analytical batch to minimise interference from time-based reactions of the investigated species ( Expected aerosol per puff yields of the analytes (assuming 100% transfer from e-liquid to aerosol) were calculated from the e-liquid concentrations of the analytes and the mass loss per puff as follows:Where W is the weight loss of the e-cigarette per puff (mg), D is the density of the e-liquid (g/ml) and C is the e-liquid concentration of the component (g/ml)

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