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Immunoblotting Cells were washed with PBS and lysed with lysis buffer (150 mM NaCl, 20 mM EDTA, 50 mM Tris-HCl, 0.5% NP-40) containing protease and phosphatase inhibitors

For example, inhibition of protein phosphatase 2 activator (PTPA) in colorectal cancer leads to activation of oncogenic pathways (e.g., MAPK, catenin beta 1 [CTNNB1]) and cellular stress responses

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The HNF4A gene maps to a region of chromosome 20 that has been linked to type 2 diabetes

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