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Large randomized controlled trials in humans are absent, so clinical efficacy claims lack robust evidence

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VGLUT1 Vesicular Glutamate Transporter 1 the universal presynaptic marker Synapsin was employed to measure juxtaposition of the newly formed spines with presynaptic boutons (Ferreira and Rapoport 2002)

In laboratory studies it has been reported as strikingly potent at promoting these connections, which is exactly why it draws so much interest and why it demands respect and supervision rather than casual use

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