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Findings from the 2014 National Youth Tobacco Survey show that current e-cigarette use (use on at least 1 day in the past 30 days) among high school students increased from 4.5% in 2013 to 13.4% in 2014, rising from approximately 660,000 to two million students

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The supernatant was removed and transferred to 68 MWCO dialysis tubing (Spectra/Por) and dialysed against ddH 2 O for 48 hours at 4 C with one water change after 24 hours

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