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Biosci Biotechnol Biochem 61:13791381

Pellets were resuspended in 300 l PBS, and 10,000 events were analysed by flow cytometry using a BD Accuri C6 Plus flow cytometer (BD Biosciences) and FCS Express 7 Research (DeNovo Software)

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Generation of KO and knockdown cell lines Optimized sgRNA oligonucleotides 64 were cloned into lentiCRISPRv2 (ref

Wheat, the model plant Arabidopsis thaliana and other C 3 photosynthesis-type plants of temperate origin are highly susceptible to drought, which causes substantial yield losses in these plants due to the effects of osmotic and oxidative stress (Gill and Tuteja 2010)