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England, L.J., et al., Developmental toxicity of nicotine: A transdisciplinary synthesis and implications for emerging tobacco products

Histology and immunofluorescence Organoids were washed to remove basement membrane extract, fixed in 4% paraformaldehyde, embedded in Histogel, paraffin-processed, sectioned (4 m) and stained according to standard hematoxylin and eosin (H&E) protocols

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(1-3) Some of tobaccos carcinogens, like tar, are specific to smoked tobacco as they occur during the combustion process of lighting a product like a cigarette, cigar, or hookah