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Primary antibodies used were tyrosine hydroxylase (Abcam cat# ab112, 1:1000), NeuN (BioLegend cat# 834501, 1:2000), alpha-synuclein (ThermoFisher cat# 32-8100, 1:1000), p-ser129 alpha-synuclein (BioLegend cat# 825702, 1:1000), calbindin D (ThermoFisher cat# MA5-50510, 1:1000), and glutamic acid decarboxylase (GAD) (ThermoFisher Cat# MA5-24909, 1:100)

Nicotine replacement may be appropriate for light, everyday smokers