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I=induced cells
cloni 10G SUPREME Electrocompetent Cells consistently outperformed “ultra high efficiency” cells from a leading supplier
2 U/µL 10X T4 DNA Ligase Buffer High Concentration Rapid Kit 1
0 × 108 145-kb BAC Clone Not tested 7 × 107 13
0 Electrocompetent Cells (> 1 × 1010 cfu/µg pKanR DNA)
E. cloni-10G CLASSIC Electrocompetent Cells, 48 rxns (SixPacks) cell projects I=induced cellsTable 1. E. cloni Competent Cells for Cloning and Library Construction E. cloni Cell Lines Transformation Efficiency (cfu g pUC DNA) Cloning Methylated DNA BAC, Cosmid Cloning Blue White Screening 10G SUPREME Electrocompetent >4 1010 YES YES YES without IPTG induction 10G ELITE Electrocompetent >2 1010 YES NO YES without IPTG induction 10G CLASSIC Electrocompetent >5 109 YES NO YES without IPTG induction 10GF ELITE Electrocompetent >2 1010 YES NO YES
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