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In the end, he became as much a prisoner of that faade as any square trapped in a nine-to-five job. The musical, which spans Thompsons childhood in Kentucky to his death in Colorado, tracks the creation of his mythologized image through his best-known works Hells Angels, The Kentucky Derby Is Decadent and Depraved, Fear and Loathing in Las Vegas, Fear and Loathing: On the Campaign Trail 72 and how his fame impacted the characters of his life: his first wife, Sandra Conklin

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Cells were resuspended in 250 L 1x Binding Buffer, and 2.5 L Annexin V-FITC and 5 L PI were added