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Furthermore, the indirect method of estimating cigarette butt densities from total anthropogenic litter introduces some uncertainty, as it may not fully capture the variation in cigarette butt accumulation, particularly in areas with distinct litter compositions or disposal patterns
The cell line was cultured in basal bronchial epithelial cell basal medium (BEBM) (Lonza, Walkersville, MD) supplemented with 2 ml of bovine pituitary extract and 0.5 ml of: insulin, hydrocortisone, retinoic acid, transferrin, triiodothyronine, epinephrine, and human recombinant epidermal growth factor (Lonza, Walkersville, MD)
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