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Determination of nicotine, nornicotine, nicotine N-oxide, cotinine, trans-3'-hydroxycotinine, and cotinine N-oxide NIC, NOR, NICNO, COT, 3HC, and COTNO in plasma (1 mL) were determined by high-performance liquid chromatography coupled with tandem mass spectrometry using deuterated cotinine as the internal standard, after prior liquidliquid extraction (pH 8, NaOH, dichloromethane-n-propanol in ratio 9:1)

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Exposure to ethanol and tobacco smoke in relation to level of PCNA antigen expression in pancreatic and hepatic rat cells