Western blotting Samples were lysed in RIPA buffer containing a protease inhibitor, and the total protein content was quantified using a bicinchoninic acid protein assay kit (Beyotime)
E4), ERK 1:1000 (137F5), KRAS 1:1000 (E2M9G), KRASG12D 1:1000 (D8H7), KRT81 1:1000 (H00003887-M01J), ZEB1 1:1000 (D80D3), E-Cadherin 1:1000 (24E10), N-Cadherin 1:1000 (D4R1H), Vimentin 1:1000 (D21H3), GATA-6 1:1000 (D61E4), SLUG 1:1000 (C19G7), SNAIL 1:1000 (C15D3), GPX4 1:1000 (E5Y8K) and Actin 1:5000 (A2066)
Glutathione peroxidase-1 reduces influenza A virus-induced lung inflammation
It plays a crucial role in fighting free radicals, reducing oxidative stress, and balancing melanin production the pigment responsible for skin color
2013, pp