van de Bospoort R, Farina M, Schmitz SK, de Jong A, de Wit H, Verhage M, Toonen RF
Research has consistently shown that glutathione levels decline with age and are depleted in many chronic disease states including Parkinson's disease, liver disease, heart disease, and alcohol use disorder
2002;132(8 Suppl):2413S-2418S
Selenotranscriptome Network in Non-alcoholic Fatty Liver Disease
Briefly, sodium bicarbonate, glucose, sodium pyruvate, and all 20 amino acids (except cystine and serine), biotin (B4639, Sigma), and vitamin B12 (V6629, Sigma) were added to the DMEM medium (#D9800-28, USBiological, Salem, MA) to match standard IMDM, supplement with 15% human AB serum and 15% dialyzed FBS (#SH300790340, Cytiva Hyclone), along with 10 ng/mL interleukin-3, 50 ng/mL stem cell factor and 2IU/mL erythropoietin