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Nevertheless, DKP is a known metabolite of AX that can arise as well by thiol-independent mechanisms, including the acidic degradation of AX ( Figure 2 Figure 3 Next, the incubation experiments were carried out in PBS buffer at 37C using different AX : DTT ratios ( Table 1 ), and the progress of the reaction was monitored by 1 H-NMR using the chemical shifts for AX -lactam ring protons (H5 and H6)