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Although ingesting small to moderate amounts of caffeine usually results in pleasant feelings, larger amounts taken all at once or within a short period of time can cause or aggravate jitteriness, insomnia (especially in caffeine abstainers), nervousness, and anxiety (Bertasi et al., 2021

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These data suggest that the anticoagulant used for sample collection and time to deproteinization, both independently and additively impact measured levels of free GSH (and thus the ratio of free GSH to GSSG) in samples of plasma which are deproteinized and stored at 80 o C

doi: 10.1080/15548627.2018.1466014 157 ZhouY.ChenZ.LiuS.LiuS.LiaoY.DuA.et al