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Baker LM, Poole LB

Plated in 96-well plates, 100,000 cells in each, were incubated 30 min at 37C with the corresponding fluorochrome: dichlorofluorescein diacetate indicated reactive oxygen species (ROS) production (485-nm excitation, 535-nm emission), MitoSOX measured mitochondrial ROS (mROS) (510-nm excitation, 580-nm emission), tetramethylrodamin methyl ester (552-nm excitation, 574-nm emission) assessed membrane potential, nonylacridin orange mitochondrial mass (495-nm excitation, 519 nm emission), and 5-chloromethylfluorescein diacetate measured intracellular glutathione (492-nm excitation, 517 nm-emission)

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G, Costamagna G

Hypoxia-inducible factors: master regulators of hypoxic tumor immune escape