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In a pilot study in Thailand, Sangsuwan and Asawanonda tested the influence of daily intake of oral collagen (5 g) for 4 weeks on skin elasticity, especially in sun-exposed areas, in a randomized, double-blind, placebo-controlled trial (in a total of 36 women, aged 4060 years, where one-half of the participants were in the treatment group [ n = 18] or the placebo group [ n = 18]) [39]

Zhang B, Yang XJ, Fang YY et al (2011) Clinical study of glutathione tablets in the treatment of hepatoblastic nuclear degeneration.Chinese Journal of New Drugs and Clinical Remedies,2011,30(03):199202

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Skin irritation is characterized by a reversible local inflammatory reaction, and MTT cell viability assay is used to estimate the damage caused by the testing irritant