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The membranes were then washed several times with water and TBS (PBS, 0.05% Tween-20), a 1 :5000 dilution of horseradish-peroxidase conjugated goat anti-rabbit antiserum was added, and the membranes further incubated for one hour at room temperature

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For instance, rat skin is thinner and has a higher density of hair follicles compared to human skin, which can lead to differences in permeability