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We have performed three neurobehavioral parameters such as open-field (for locomotion), T-maze (for memory), and novel diving test (for anxiety), followed by biochemical parameters such as total protein estimation, LPO, GSH, nitrite, AChEs, and catalase, neurotransmitters (GABA and glutamate) along with inflammatory markers (IL-1, TNF-, and IL-17), and histopathological evaluation at the end

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The enzyme methylenetetrahydrofolate reductase (MTHFR) then turns 5, 10 Methylene THF into L-Methylfolate (5-MTHF)