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The COCs were then homogeneously distributed in 4-well plates with 500 L of maturation medium: TCM199 and 4 L/mL gentamicin, 100 M cysteamine, 10 g/mL follicle-stimulating hormone (FSH), 10 g/mL luteinizing hormone (LH), and 10% fetal calf serum (FCS)

In addition, to allay the concern that results could reflect emergence of mutants in the population, we assayed heritability of the high-GSH phenotype following culture with D-HIS

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