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Empty capsids that co-eluted with full capsids from 34C infection temperature harvests were poorly resolved across the conditions tested, but subsequent polishing anion exchange and cation exchange chromatography steps were developed to clear residual empty capsids and other impurities

Interestingly, another study using the lung cancer model observed a downregulated expression of 26S proteasome in lung cancer stem cells

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In addition, a good balance of bile acids and an increase in their secretion are essential