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ECs were incubated overnight with the supernatant at 37C, followed by the addition of 10 g/ml polybrene

Genetic advances and functional characterizations of the pathophysiological alterations in receptor channel trafficking and function are changing our understanding of IGEs showed clear that genetic mutation of transmembrane ion channels is the underlying cause of many forms of human IGEs (ONeill et al

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In contrast, the ALP activity of the cells treated with 1, 5, or 10 mmol/L LC had no difference compared with that of the calcifying controls