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One hundred microliters of a 50% slurry of prewashed protein G-agarose beads (GE Healthcare) was then added to each sample, followed by incubation for an additional 4 h at 4C

To further verify that the decreased resistance in the hr-52 mutant is caused by the interference with AT4G23100 gene, we obtained the previously reported mutant pad2-1 with the Ser298Asn substitution (S298N) within AT4G23100 21 to assess its responses to B

Chalcones are known to exhibit diverse therapeutic and pharmacological activities such as antioxidant and antiinflammatory

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