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The absence of post-1992 human data is not neutral

However, due to experimental constraints, the concentration of metformin used was relatively high, making it challenging to apply in clinical treatments for patients [28]

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After 2 days in vitro (DIV), the cultures were changed to Prenatal Mouse Neuron Media (PMNM) composed of Neurobasal media (Gibco/Fisher Scientific, Cat# 21-103-049) supplemented with B-27 Plus (Fisher Scientific, Cat# A3582801), GlutaMAX Supplement, and penicillin/streptomycin