Abbreviations APC: Adenomatous polyposis coli CDK5RAP2: Cyclin-dependent kinase 5-related activator protein 2 EB1: End-binding protein-1 ERK: Extracellular signal-regulated kinase CLASP: Cytoplasmic linker protein (CLIP)-associated protein FAK: Focal adhesion kinase GCP: -tubulin complex protein GSK-3: Glucogen synthase kinase-3 ILK: Integrin-linked kinase IQGAP: IQ motif-containing GTPase-activating protein KANK: KN motif and ankyrin-repeat-containing protein KIF21A: Kinesin family member 21A LL5: Pleckstrin homology-like domain family B member 2 mDIA: Diaphanous-related formin-1 MMP: Matrix metalloproteinase MOZART: Mitotic spindle organizing protein associated with a ring of -tubulin NEDD1: Neural precursor cell expressed, developmentally down-regulated-protein 1 PAK: p21-activated kinase PAR6: Partitioning-defective-6 PKC: Protein kinase C RSK2: Ribosomal-S6 kinase-2 References Hynes RO (2002) Integrins: bidirectional, allosteric signaling machines
Acknowledgements We thank Lauren Knightley, Beth Jago, Meirion Richards, and Amin Mirza of the Structural Chemistry team for their expertise and assistance, Swen Hoelder for his continuous support, Stephen Hearnshaw for his contributions to crystal optimisation, and Chris Richardson for crystallography computing support
Important in DNA synthesis, vitamin B-12 is bound to the protein in food, and is released by stomach acids during digestion
This is especially suspect if nothing else in your routine has changed
After reconstitution, aliquot and store at 20 C to preserve peptide structural integrity
