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The definitive way to confirm a peptide is laboratory analysis: high-performance liquid chromatography (HPLC) measures purity, mass spectrometry (MS) confirms molecular identity, and a Limulus Amebocyte Lysate (LAL) assay screens for bacterial endotoxins

This creates a net negative balance within adipocytes, resulting in decreased lipid droplet size and eventual adipocyte shrinkage

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Levels of the target transcript were normalized to a GAPDH endogenous control, constantly expressed in both groups (Ct)