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Shaking can shear and destroy the delicate peptide chains

Transepithelial Electrical Resistance and Paracellular Permeability Currently, there are different commercially available Ussing chamber equipments that are designed to accommodate multiple epithelial sources such as cell cultures grown in membranes, animal tissue and human tissue from resection specimens or endoscopic biopsies

Cancer 11 , 325337 (2011)

The 10 mg/mL concentration has become the standard for good reason, and the peptide reconstitution calculator at SeekPeptides can verify your math regardless of which concentration you choose