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The FDA prescribing information explicitly warns against taking vitamin A supplements alongside isotretinoin because of the risk of additive hypervitaminosis A toxicity, which can produce symptoms including pseudotumor cerebri (raised intracranial pressure), hepatotoxicity, and bone changes [1]

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When calculated from the concentrations measured by HF-LPME (pH 12 acceptor phase), the EC50 ranged from 223.4 to 394.6 mg L 1 , and the CV decreased to 27.60%, suggesting that the concentrations measured by HF-LPME can be used to estimate the toxicity of sulfadiazine irrespective of the solution pH