The total protein concentration was detected by Enzyme Label Detector (#Epoch, BioTek Instruments, Inc., headquartered in Winooski, VT, USA)
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Hepatic taurine synthesis is largely restricted by the low availability of cysteinesulfinate as substrate for cysteinesulfinate decarboxylase, and taurine production is increased when cysteinesulfinate increases in response to an increase in the hepatic cysteine concentration and the associated increase in cysteine dioxygenase activity (Stipanuk 2004)
Therefore, regimens of a loading phase followed by a maintenance phase appear to be effective in increasing total intramuscular creatine levels in rats