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Email: [email protected] ORCID: Affiliation: 16 Centro de Investigacin Biomdica en Red Enfermedades Neurodegenerativas (CiberNed), National Institute of Health Carlos iii, 28031 Madrid, Spain 17 Departament de Biochemistry and Molecular Biomedicine, University of Barcelona, 08028 Barcelona, Spain 18 Institut de Qumica Terica i Computacional (IQTCUB), School of Chemistry, University of Barcelona, 08028 Barcelona, Spain These authors contributed equally to this work

Stock buffer (96.6l of 125mM sodium phosphate, 6.3mM disodium EDTA, pH 7.5) was then added to each tube, vortexed and re-centrifuged as above

if so, how long did it take

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