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Apoptosis of Esophageal Cancer Cells following Ad-TNFa gene therapy: the role of the double-stranded RNA activatedprotein kinase (PKR) and the nuclear factor kappa B (NF-kB)

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Isotopic labeling studies and metabolic flux analysis are particularly useful for this purpose, as they allow researchers to track the movement of labeled metabolites through interconnected pathways

For analysis of genes expressed, TaqMan probes for human IFNG , IFITM3 , IFI27 , IFI44L , IFIT1 , MX1 , ISG15 , MX2 , RSAD2 and SIGLEC1 , mouse Ndusf8 , Sdha , Sdhb , Sdhc , Txnrd1 , Got1 , Arg1 , Tgfb1 , Ccl2 , Ccl3 , Fn1 , Mmp2 and Mmp12 or viral N1 and N2 (SARS-CoV-2) (IDT) were used