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Indirect damage, accounting for two-thirds of the radiation-induced cell injury, means ionizing radiation is able to react with substances in cells such as water to form toxic ROS (Hall et al., 2016)

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After more vigorous pipetting, cells were pelleted by centrifugation at 400 RCF for 5 min and seeded onto a 24-well plate

The losses of the fortificant were further markedly decreased when it was co-encapsulated along with sodium ascorbate, which enhances resistance of 5-methyl-THF to thermal oxidative degradation as reported above 431

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