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Several studies have revealed that the gene encoding 3-phosphoglycerate dehydrogenase, the rate-limiting serine biosynthesis enzyme, is amplified in breast cancers and melanomas [25,26]

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This endogenous nature distinguishes GHK-Cu from many synthetic peptides and is one reason for the broad research interest

however, several studies reported marked changes in outer hair cell death when jet fuel and noise exposures were combined

The positive transformants were selected on YPD plates with addition of antibiotics geneticin (G418, 4 mg/mL) or hygromycin B (HygB, 1 mg/mL) and verified by DNA sequencing after the integrated gene fragments were amplified by PCR using the corresponding primers and the genomic DNA templates