Some older human studies explored DSIP in people with sleep issues and suggested possible effects on sleep structure or daytime function
Samples were reduced with DTT and alkylated with iodoacetamide as described above, then diluted with DPBS (300 L), and taken forward to LysC and trypsin digestion without precipitation
Plain sterile water is acceptable only for single-use experiments because there is no preservative for the multi-week draw window
Here is a side-by-side breakdown to help you decide
Ternant D, Ducourau E, Fuzibet P, Vignault C, Watier H, Lequerre T, Le Loet X, Vittecoq O, Goupille P, Mulleman D, et al