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Cell Death Dis (2021) 12(2):160

Mutation of certain proximal residues did result in loss of activity (N78 and R130 discussed below)

doi: 10.1080/14740338.2017.1269168 99 Almeida-BrasilCCHanlyJGUrowitzMClarkeAERuiz-IrastorzaGGordonCet al

Binding of free insulin to the cell membrane-bound IR results in activation of the intrinsic tyrosine kinase of insulin receptor (IR) then phosphorylates the -subunit of IR which stimulates activation of the insulin signaling cascade through PI3K and AKT, ultimately leading to translocation of the glucose transporter 4 to the cell membrane of skeletal muscle and adipose cells and uptake of glucose (Styskal et al., 2012)