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Ecotoxicol Environ Saf 139:210218 Aqeel U, Parwez R, Aftab T, Khan MMA, Naeem M (2022) Exogenous calcium repairs damage caused by nickel toxicity in fenugreek (Trigonella foenum-graecum L.) by strengthening its antioxidant defense system and other functional attributes

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Preparation of Cell Free Extract Yeast cells grown in Sabouraud dextrose broth were harvested at 72 h of growth by centrifugation at 7,000x g washed with 0.5 M of potassium phosphate buffer, pH 7.5 containing phenylmethylsulfonyl fluoride (1 mM), EDTA (4 mM) and 2-mercaptoethanol (5 mM) and resuspended in the same buffer

Notably, when CPT1A was knocked down, it resulted in enhanced ubiquitination of the c-Myc protein and a decreased binding of c-Myc protein to CPT1A (Fig

Kumar S, Cakouros D