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Genes Isolation, Promoter Cloning, and Plasmid Construction The full-length coding sequences (CDS) of all the genes utilized in this study were amplified from the Biqi cDNA and cloned into the pEASY-Blunt Simple Cloning Vector (TranGen, Beijing, China) for sequencing

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Lipid Panel Frequency The ACC/AHA 2019 guideline on the management of blood cholesterol recommends a fasting lipid panel 4 to 12 weeks after starting a PCSK9 inhibitor, then every 3 to 12 months thereafter to confirm therapeutic response

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