Cells were washed once with PBS and processed for cell cycle analysis as described [28]
Diallyl disulfide (DADS) consists of a disulfide bond that is more reactive than DAS, whereas diallyl trisulfide (DATS), a complex structure of three sulfur atoms, is more reactive due to the presence of multiple sulfur atoms, which increases its capacity to engage in redox reactions (156)
Multiple studies have shown that diabetes is associated with cognitive impairment, in which HO-1 as an important antioxidant enzyme, plays an important role in protecting against apoptosis, inflammation, and oxidative stress in diabetic cognitive dysfunction [162,163,164]
When free radicals outnumber antioxidants, the resulting imbalance is known as oxidative stress
Ann Rheum Dis (1990) 49(12):9802.10.1136/ard.49.12.980 158 BergstromUJacobssonLNilssonJBerglundGTuressonC