We thank the Lille University Foundation, FINOVAM, FIRA, Nord France Amorage, BPI France and the region Hauts-de-France for funding InBrain Pharma
The supernatant of protein extracts was heated at 70 C for 10 min in SDS loading buffer (2% (w/v) SDS, 10% (w/v) glycerol, 0.01% (w/v) bromophenol blue and 0.125 M Tris-HCl, 0.3 M DTT, pH 6.8) and loaded on SDS-PAGE acrylamide gels for western blotting
To assess small intestinal transit, we employed an activated carbon gavage test 17 , which revealed a significant, dose-dependent reduction in the small intestinal transit rate in morphine-treated mice compared to saline-treated controls (Fig
They have shockingly little nutritional value, but were filling up on these foods
we discuss this at consultation