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It should be kept away from strong chelators (such as high levels of EDTA and related sequestrants), reducing sugars, thiol-bearing actives, and high concentrations of direct acids (for example ascorbic acid or alpha-hydroxy acids at low pH), all of which can compete for the copper center or disrupt the peptide-metal bond and shift the characteristic blue color toward brown or colorless degradation products
Glutathione is composed of three amino acids, cysteine, glycine, and glutamine
This basal medium was then augmented with a specific cocktail of small molecules: 3 M or 1 M CHIR99021 (CHIR), IDE1, 2 M Ly294002 (Ly), and 0.75 M PD0332991 (PD)
Copper ions in GHK-Cu are highly susceptible to atmospheric oxidation, with a half-life of only 4.2 hours in aqueous solutions at pH 6.5