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One hundred microlitres of tenfold serial dilutions of EV-F3 in serum-free G-MEM was added to washed, confluent cells

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The medium was concentrated using 0.5 mL Amicon Ultra 10 kDa molecular weight cut-off filters (Millipore, Ireland), in accordance with the manufacturers instructions

The 9-h and 24-h DHA treatments similarly persisted in altering ribosome synthesis, protein-DNA complex assembly, proteasome activity, proteasomal protein catabolism, immune responses, cytoplasmic translation, peptide biosynthesis, and ribosome biogenesis

CAIS x CAIL: Day-to-Night Copper Support We recommend applying Copper Amino Isolate Serum in the morning and Copper Amino Isolate Lipid 1% in your evening regimen for a more versatile approach, supporting innate collagen and targeting visible signs of ageing including the look of elasticity, volume and skin tone