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Quantitative PCR To analyze mRNA expression, total RNA was collected from cells using the PureLink RNA Mini Kit (Thermo Fisher Scientific), and 1 g was reverse-transcribed using the ProtoScript II First Strand cDNA Synthesis Kit with oligo(dT) primers and murine RNase inhibitor (New England Biolabs)

Western blot analysis revealed the hepatic LDLR expression levels

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WCBs offer many advantages, including the low cost of growing small quantities of bacterial culture and access to a range of configurable and orthogonal output signal detection modalities, including luminescence, fluorescence and colorimetric approaches

Both authors read and approved the final version of the manuscript