The 2025 IV pilot measured cardiac, hepatic, renal, thyroid, and glucose biomarkers and reported no abnormalities (Lee et al., 2025), which gives a sense of what those investigators considered worth checking
On the other hand, a severe B12 deficiency can also lead to weight loss
Theoretical considerations include: Potential interactions requiring monitoring: Other fat-loss agents or thermogenics (additive effects), growth hormone or growth hormone secretagogues (mechanism overlap), medications affecting glucose metabolism (monitor blood sugar despite AOD-9604s neutral glycemic effects)
Mistake 4: storing at room temperature Some researchers leave their vial on the bathroom counter or bedroom nightstand for convenience
Target 0.7 to 1.0 grams of protein per pound of body weight daily