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Females of childbearing potential must use two reliable forms of contraception simultaneously during oral tretinoin therapy and for one month following discontinuation of therapy, unless abstinence is the chosen method

ROS and lipid peroxidation assay Day 12 cultured SCD erythroblasts under different treatments were incubated with 5 M 2-7-dichlorodihydrofluorescein diacetate (H2DCFDA) (#D6883, Sigma) for 30 min at 37 C, washed with PBS, and analyzed on a BD LSR-II flow cytometer for ROS level

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If recovery improves and movement becomes easier, physical activity often increases naturally