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The activated iodine binds to tyrosine residues on thyroglobulin molecules under the action of TPO to produce monoiodotyrosine (MIT) and diiodotyrosine (DIT), which are subsequently coupled to produce T3 or T4 ( Figure 1 )

Halogen facilitates the binding with the enzyme because it increases the binding affinity and binding selectivity, and adding halogens to lead during drug development is well recognized to be a general optimizing approach [31]

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