The cell cycle was studied by flow cytometry, using the level of the fluorescence of the DNA dye propidium iodide (final concentration, 5 g/mL) at 630 nm fluorescence emission as a measure of the DNA content per cell, as described in materials and methods
Exploratory maternal and cord blood metabolomics approaches to sulfur pathways Secondary metabolomics analyses highlighted significant differences in metabolites and enzyme activity indexes in maternal and cord blood depending on NAC-treatment with separation between severe and moderate IPTB forms (whether such differences were explained by severity per se or by the fact that the iv route of NAC delivery is used in severe cases, is unclear), and as a function of gestational age (all data are represented in Supplementary Table S5 and main results are illustrated in Fig
This was corroborated by the marked reduction in the water content of R
The regulatory environment for actually collecting data and running trials on them
The subsequent antigen presentation triggers the activation of Th1 and Th17 cells, leading to the release of pro-inflammatory cytokines (IL-2, TNF-, IFN-, IL-17), which in turn amplify oxidative stress and perpetuate an amplifying loop of inflammation and tissue damage (Kubiak et al
