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The following antibodies were purchased from Cell Signaling Technology: Met (25H2) mouse mAb, phospho-Met (Tyr1234/1235) (D26) XP rabbit mAb, Akt (11E7) rabbit mAb, phospho-Akt (Ser473) (D9E) XP rabbit mAb, Erk1/2 (137F5) rabbit mAb, phospho-Erk1/2 (Thr202/Tyr204) (D13.14.4E) XP rabbit mAb, Gab1 rabbit mAb, phospho-Gab1 (Tyr 627) (C32H2) rabbit mAb and phospho-EGFR (Tyr 1068) rabbit polyclonal Ab

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Conversely, following hypoxia or loss of mitochondrial membrane potential, the inactivation or reduction of tyrosine kinase activity through the activation of PGAM5 will contribute to the downregulation of the phosphorylated FUNDC1 level