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52333003 to X.J.), Basic Research Program of Jiangsu (BK20244003) and the Natural Science Foundation of Jiangsu Province (BK20202002)

After incubation for 10 min, 200 L luciferin solution (containing 20 mM tricine, 1.0 mM MgSO4, 0.1 mM EDTA, 33 mM dithiothreitol, 270 M coenzyme A, 500 M D-luciferin, and 530 M ATP, pH 7.8) was added, and then the firefly luminescence was measured with a BioTek synergy 4 Multi-Mode Microplate Reader

The documented cycle is 812 weeks on (loading through maintenance) followed by 48 weeks off before any repeat cycle

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Although tobacco carcinogen metabolites have been found in the urine and blood of never-smoking individuals exposed to SHS 253 , large-scale genomic studies of LCINS have shown that reported SHS exposure often does not correlate with smoking-related somatic mutational signatures ,7,78